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DIRAS3 Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DIRAS3 Antibody AP5892a
    Western blot analysis of lysates from Hela cell line and human ovary tissue lysate (from left to right), using DIRAS3 Antibody(Cat. #AP5892a). AP5892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • WB - DIRAS3 Antibody AP5892a
    DIRAS3 Antibody (Cat. #AP5892a) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DIRAS3 antibody detected the DIRAS3 protein (arrow).
    detail
  • IHC-P - DIRAS3 Antibody AP5892a
    DIRAS3 Antibody (Cat. #AP5892a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DIRAS3 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - DIRAS3 Antibody AP5892a
    DIRAS3 Antibody (Cat. #AP5892a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O95661
Other Accession NP_004666.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25861 Da
Additional Information
Gene ID 9077
Other Names GTP-binding protein Di-Ras3, Distinct subgroup of the Ras family member 3, Rho-related GTP-binding protein RhoI, DIRAS3, ARHI, NOEY2, RHOI
Target/Specificity This DIRAS3 Antibody is generated from rabbits immunized with recombinant protein of human DIRAS3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDIRAS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DIRAS3
Synonyms ARHI, NOEY2, RHOI
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers
Research Areas
Citations (0)
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$ 365.00
$ 140.00
Cat# AP5892a
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