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CUX1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CUX1 Antibody (C-term) AP5895B
    All lanes : Anti-CUX1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 164 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CUX1 Antibody (C-term) AP5895B
    All lanes : Anti-CUX1 Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 164 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CUX1 Antibody (C-term) AP5895B
    CUX1 Antibody (C-term) (Cat. #AP5895b) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - CUX1 Antibody (C-term) AP5895B
    Fluorescent confocal image of Hela cell stained with CUX1 Antibody (C-term)(Cat#AP5895b).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CUX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CUX1 immunoreactivity is localized to Nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P39880
Other Accession NP_853530.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 164187 Da
Antigen Region 1347-1374 aa
Additional Information
Gene ID 1523
Other Names Homeobox protein cut-like 1, CCAAT displacement protein, CDP, Homeobox protein cux-1, CUX1, CUTL1
Target/Specificity This CUX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1347-1374 amino acids of human CUX1.
Dilution WB~~1:2000
IHC-P~~1:50~100
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCUX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CUX1
Synonyms CUTL1
Function Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity).
Cellular Location Nucleus.
Citations ( 0 )
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$ 295.00
$ 99.00
Cat# AP5895B
Size:
Quantity:
(40 western blots)
Availability: In Stock
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