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ESRP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - ESRP1 Antibody  (N-term) AP5940a
    AP5940a staining ESRP1 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - ESRP1 Antibody  (N-term) AP5940a
    All lanes : Anti-ESRP1 Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ESRP1 Antibody  (N-term) AP5940a
    All lanes : Anti-ESRP1 Antibody (N-term) at 1:2000 dilution Lane 1: HCT116 whole cell lysates Lane 2: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ESRP1 Antibody  (N-term) AP5940a
    ESRP1 Antibody (N-term) (Cat. #AP5940a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESRP1 antibody detected the ESRP1 protein (arrow).
  • IHC-P - ESRP1 Antibody  (N-term) AP5940a
    ESRP1 antibody (N-term) (Cat. #AP5940a) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ESRP1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q6NXG1
Other Accession B2RYD2, Q3US41, NP_001116298.1, NP_060167.2, NP_001030087.2, NP_001116299.1, NP_001116297.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 75585 Da
Antigen Region 182-211 aa
Additional Information
Gene ID 54845
Other Names Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A, RNA-binding protein 35A, ESRP1, RBM35A
Target/Specificity This ESRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids of human ESRP1.
Dilution IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsESRP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESRP1
Synonyms RBM35A
Function mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Cellular Location Nucleus.
Tissue Location Epithelial cell-specific.
Citations ( 0 )
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$ 295.00
$ 99.00
Cat# AP5940a
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Quantity:
(40 western blots)
Availability: In Stock
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