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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ABCB10 Antibody (N-term)   

ABCB10 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - ABCB10 Antibody (N-term) AP6109a
    AP6109a staining hABCB10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - ABCB10 Antibody (N-term) AP6109a
    All lanes : Anti-ABCB10 Antibody (S16) at 1:1000-1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 79 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ABCB10 Antibody (N-term) AP6109a
    ABCB10 Antibody (S16) (Cat. #AP6109a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ABCB10 antibody detected the ABCB10 protein (arrow).
  • IHC-P - ABCB10 Antibody (N-term) AP6109a
    ABCB10 antibody (N-term) (Cat. #AP6109a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ABCB10 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NRK6
Other Accession NP_036221
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79148 Da
Antigen Region 1-27 aa
Additional Information
Gene ID 23456
Other Names ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10, ABC transporter 10 protein, Mitochondrial ATP-binding cassette 2, M-ABC2, ABCB10
Target/Specificity This ABCB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human ABCB10.
Dilution IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB10
Function May mediate critical mitochondrial transport functions related to heme biosynthesis.
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Tissue Location Ubiquitous. Highly expressed in bone marrow, expressed at intermediate to high levels in skeletal muscle, small intestine, thyroid, heart, brain, placenta, liver, pancreas, prostate, testis, ovary, leukocyte, stomach, spinal cord, lymph node, trachea and adrenal gland, and low levels are found in lung, kidney, spleen, thymus and colon
Research Areas
Citations (0)

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The membrane-associated protein ABCB10 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown.


Zhang, F., et al., J. Biol. Chem. 275(30):23287-23294 (2000). Allikmets, R., et al., Mamm. Genome 6(2):114-117 (1995). Zhang, F., et al., FEBS Lett. 478 (1-2), 89-94 (2000).

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Cat# AP6109a
(40 western blots)
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