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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ABCB4 Antibody (Center)   

ABCB4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ABCB4 Antibody (Center) AP6112A
    ABCB4-S639 (Cat. #AP6112a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ABCB4 antibody detected ABCB4 protein (arrow).
  • IHC-P - ABCB4 Antibody (Center) AP6112A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P21439
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 141523 Da
Antigen Region 624-654 aa
Additional Information
Gene ID 5244
Other Names Multidrug resistance protein 3, ATP-binding cassette sub-family B member 4, P-glycoprotein 3, ABCB4, MDR3, PGY3
Target/Specificity This ABCB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 624-654 amino acids from the Central region of human ABCB4.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB4 (HGNC:45)
Function Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to fu rther enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP- dependent manner (PubMed:24045840). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (By similarity).
Cellular Location Cell membrane; Multi- pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Apical cell membrane; Multi- pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Membrane raft. Cytoplasm. Cytoplasmic vesicle, clathrin- coated vesicle {ECO:0000250|UniProtKB:Q08201}. Note=Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity) Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA- dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of canalicular plasma membranes (PubMed:23468132). {ECO:0000250|UniProtKB:P21440, ECO:0000269|PubMed:15258199, ECO:0000269|PubMed:19674157, ECO:0000269|PubMed:23468132}
Research Areas
Citations ( 0 )

Background

The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.

References

Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 105(2):132-135 (2002).
Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 104(2):109-112 (2002).
Jacquemin, E., Semin. Liver Dis. 21(4):551-562 (2001).
Smit, J.J., et al., Biochim. Biophys. Acta 1261(1):44-56 (1995).
Ruetz, S., et al., Cell 77(7):1071-1081 (1994).

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Discontinued
Cat# AP6112A
Size:
(40 western blots)
Alternative Products:

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