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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   HAND2 Antibody (C-term)   

HAND2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HAND2 Antibody (C-term) AP6117a
    Western blot analysis of anti-HAND2 Antibody (C-term) (Cat.#AP6117a) in NCI-H460 cell line lysates (35ug/lane). HAND2(arrow) was detected using the purified Pab.
  • IHC-P - HAND2 Antibody (C-term) AP6117a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P61296
Other Accession P61295, Q61039, P57102, NP_068808
Reactivity Human
Predicted Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23666 Da
Antigen Region 179-207 aa
Additional Information
Gene ID 9464
Other Names Heart- and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, bHLHa26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2, dHAND, HAND2, BHLHA26, DHAND
Target/Specificity This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-207 amino acids from the C-terminal region of human HAND2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHAND2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAND2
Synonyms BHLHA26, DHAND
Function Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3' (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981}
Tissue Location Heart.
Research Areas
Citations (0)

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Background

HAND2 belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.

References

McFadden, D.G., et al., Development 127(24):5331-5341 (2000). Firulli, A.B., et al., Nat. Genet. 18(3):266-270 (1998). Russell, M.W., et al., Biochim. Biophys. Acta 1443(3):393-399 (1998). Srivastava, D., Trends Cardiovasc Med 9 (1-2), 11-18 (1999). Srivastava, D., et al., Cold Spring Harb. Symp. Quant. Biol. 67, 121-125 (2002).

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$ 295.00
$ 99.00
Cat# AP6117a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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