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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ABCB5 Antibody (N-term)   

ABCB5 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ABCB5 Antibody (N-term) AP6122a
    Western blot analysis of ABCB5 Antibody (N-term) (Cat.#AP6122a) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.
  • FC - ABCB5 Antibody (N-term) AP6122a
    Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
  • FC - ABCB5 Antibody (N-term) AP6122a
    Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
  • SPECIFICATION
  • CITATIONS: 5
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q2M3G0
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 138641 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 340273
Other Names ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5
Target/Specificity This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB5
Function Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:12960149}
Tissue Location Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.
Research Areas
Citations ( 0 )

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

References

Frank,N.Y., Cancer Res. 65 (10), 4320-4333 (2005) Chen,K.G., Pigment Cell Res. 18 (2), 102-112 (2005) Frank,N.Y., J. Biol. Chem. 278 (47), 47156-47165 (2003)

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$ 295.00
$ 99.00
Cat# AP6122a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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