|Application ||IHC-P, WB, FC, E|
|Calculated MW||22625 Da|
|Antigen Region||156-185 aa|
|Other Names||Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278, ICOS, AILIM|
|Target/Specificity||This ICOS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the C-terminal region of human ICOS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ICOS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
ICOS belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Okamoto, N., et al., Biochem. Biophys. Res. Commun. 310(3):691-702 (2003).
Okamoto, T., et al., J. Rheumatol. 30(6):1157-1163 (2003).
Riley, J.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(18):11790-11795 (2002).
Haimila, K.E., et al., Immunogenetics 53(12):1028-1032 (2002).
Witsch, E.J., et al., Eur. J. Immunol. 32(9):2680-2686 (2002).
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