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MAGEA9 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAGEA9 Antibody (Center) AP6170a
    The anti-MAGEA9 Ctr Antibody (Cat.#AP6170a) is used in Western blot to detect MAGEA9 in Placenta lysate.
  • IHC-P - MAGEA9 Antibody (Center) AP6170a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - MAGEA9 Antibody (Center) AP6170a
    Western blot analysis of MAGEA9 (arrow) using rabbit polyclonal MAGEA9 Antibody (C185) (Cat.#AP6170a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P43362
Other Accession NP_005356
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35088 Da
Antigen Region 171-198 aa
Additional Information
Gene ID 4108;728269
Other Names Melanoma-associated antigen 9, Cancer/testis antigen 19, CT19, MAGE-9 antigen, MAGEA9, MAGE9, MAGEA9A
Target/Specificity This MAGEA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human MAGEA9.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAGEA9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAGEA9
Synonyms MAGE9, MAGEA9A
Function Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue Location Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta
Research Areas
Citations ( 0 )

Background

MAGEA9 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.

References

Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

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$ 295.00
$ 99.00
Cat# AP6170a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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