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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   MAGEF1 Antibody (C-term)   

MAGEF1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAGEF1 Antibody (C-term) AP6179a
    The anti-MAGEF1 C-term Antibody (Cat.#AP6179a) is used in Western blot to detect MAGEF1 in A375 lysate.
  • IHC-P - MAGEF1 Antibody (C-term) AP6179a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9HAY2
Other Accession NP_071432
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 274-303 aa
Additional Information
Other Names Melanoma-associated antigen F1, MAGE-F1 antigen, MAGEF1
Target/Specificity This MAGEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the C-terminal region of human MAGEF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAGEF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAGEF1 (HGNC:29639)
Function Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair (PubMed:29225034).
Tissue Location Ubiquitous.. EMBL; AF295378; AAG30208.1; -; mRNA EMBL; AC107294; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC010056; AAH10056.1; -; mRNA EMBL; AF320910; AAG38606.1; -; mRNA CCDS; CCDS3269.1; - RefSeq; NP_071432.2; NM_022149.4 UniGene; Hs.306123; - ProteinModelPortal; Q9HAY2; - SMR; Q9HAY2; - BioGrid; 122067; 12 IntAct; Q9HAY2; 6 STRING; 9606.ENSP00000315064; - iPTMnet; Q9HAY2; - PhosphoSitePlus; Q9HAY2; - BioMuta; MAGEF1; - DMDM; 300669649; - EPD; Q9HAY2; - PaxDb; Q9HAY2; - PeptideAtlas; Q9HAY2; - PRIDE; Q9HAY2; - ProteomicsDB; 81457; - DNASU; 64110; - Ensembl; ENST00000317897; ENSP00000315064; ENSG00000177383 GeneID; 64110; - KEGG; hsa:64110; - UCSC; uc003fpa.4; human CTD; 64110; - EuPathDB; HostDB:ENSG00000177383.4; - GeneCards; MAGEF1; - H-InvDB; HIX0003923; - HGNC; HGNC:29639; MAGEF1 HPA; HPA046899; - HPA; HPA054190; - MIM; 609267; gene neXtProt; NX_Q9HAY2; - OpenTargets; ENSG00000177383; - PharmGKB; PA134920978; - eggNOG; KOG4562; Eukaryota eggNOG; ENOG4111S70; LUCA GeneTree; ENSGT00760000118824; - HOGENOM; HOG000231161; - HOVERGEN; HBG006315; - InParanoid; Q9HAY2; - OMA; GYPKRLI; - OrthoDB; EOG091G0TJ0; - PhylomeDB; Q9HAY2; - TreeFam; TF328505; - ChiTaRS; MAGEF1; human GenomeRNAi; 64110; - PRO; PR:Q9HAY2; - Proteomes; UP000005640; Chromosome 3 Bgee; ENSG00000177383; - CleanEx; HS_MAGEF1; - Genevisible; Q9HAY2; HS GO; GO:2000042; P:negative regulation of double-strand break repair via homologous recombination; IDA:UniProtKB GO; GO:2000060; P:positive regulation of ubiquitin-dependent protein catabolic process; IDA:UniProtKB GO; GO:0097428; P:protein maturation by iron-sulfur cluster transfer; IDA:UniProtKB GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB InterPro; IPR037445; MAGE InterPro; IPR002190; MHD_dom PANTHER; PTHR11736; PTHR11736; 1 Pfam; PF01454; MAGE; 1 SMART; SM01373; MAGE; 1 PROSITE; PS50838; MAGE; 1 1: Evidence at protein level; Complete proteome; Polymorphism; Reference proteome; Tumor antigen; Ubl conjugation pathway CHAIN 1 307 Melanoma-associated antigen F1 /FTId=PRO_0000156732 DOMAIN 76 277 MAGE. {ECO:0000255|PROSITE- ProRule:PRU00127} VARIANT 93 93 K -> R (in dbSNP:rs34540780) /FTId=VAR_057651 MUTAGEN 87 88 Missing: Loss of interaction with NSMCE1 CONFLICT 66 66 A -> S (in Ref. 1; AAG30208, 2; AAG38606 and 3; AAH10056). CONFLICT 158 158 E -> EE (in Ref. 1; AAG30208, 2; AAG38606 and 3; AAH10056). CONFLICT 236 236 E -> A (in Ref. 1; AAG30208, 2; AAG38606 and 3; AAH10056). SEQUENCE 307 AA; 35222 MW; 8CB673B3FF7921EE CRC64; MLQTPESRGL PVPQAEGEKD GGHDGETRAP TASQERPKEE LGAGREEGAA EPALTRKGAR ALAAKALARR RAYRRLNRTV AELVQFLLVK DKKKSPITRS EMVKYVIGDL KILFPDIIAR AAEHLRYVFG FELKQFDRKH HTYILINKLK PLEEEEEEDL GGDGPRLGLL MMILGLIYMR GNSAREAQVW EMLRRLGVQP SKYHFLFGYP KRLIMEDFVQ QRYLSYRRVP HTNPPEYEFS WGPRSNLEIS KMEVLGFVAK LHKKEPQHWP VQYREALADE ADRARAKARA EASMRARASA RAGIHLW
Research Areas
Citations (0)

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Background

Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGE is unknown, may play a role in embryonal development and tumor transformation or aspects of tumor progression.

References

Stone, B., et al., Gene 267(2):173-182 (2001).

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$ 295.00
$ 99.00
Cat# AP6179a
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(40 western blots)
Availability: In Stock
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