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MAGEL2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UJ55 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 132822 Da |
| Antigen Region | 499-529 aa |
| Gene ID | 54551 |
|---|---|
| Other Names | MAGE-like protein 2, Necdin-like protein 1, Protein nM15, MAGEL2, NDNL1 |
| Target/Specificity | This MAGEL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-529 amino acids from the C-terminal region of human MAGEL2. |
| Dilution | IHC-P~~1:10~50 WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAGEL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAGEL2 |
|---|---|
| Synonyms | NDNL1 |
| Function | Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (PubMed:23452853). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (By similarity). |
| Cellular Location | Early endosome. Cytoplasm {ECO:0000250|UniProtKB:Q9QZ04}. Nucleus {ECO:0000250|UniProtKB:Q9QZ04}. Note=Recruited to retromer-containing endosomes via interaction with VPS35. Colocalizes with CLOCK and BMAL1 in the cytoplasm, and with PER2 in the cytoplasm and nucleus (By similarity). {ECO:0000250|UniProtKB:Q9QZ04, ECO:0000269|PubMed:23452853} |
| Tissue Location | Expressed in placenta, fetal and adult brain. Not detected in heart and small intestine, very low levels in fibroblasts Not expressed in brain of a Prader-Willi patient |
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Provided below are standard protocols that you may find useful for product applications.
Background
Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGE is unknown, may play a role in embryonal development and tumor transformation or aspects of tumor progression.
References
Lee, S., et al., Hum. Mol. Genet. 9(12):1813-1819 (2000).
Boccaccio, I., et al., Hum. Mol. Genet. 8(13):2497-2505 (1999).
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