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AF9 (MLLT3) Antibody (Center V422)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6190b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
AF9 (MLLT3) Antibody (Center V422) - Product info | |
| Application | WB
|
| Primary Accession | P42568 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 63351 Da |
AF9 (MLLT3) Antibody (Center V422) - Additional info | |
| Gene ID 4300 | |
| Other Names MLLT3; AF9; YEATS3; Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3 | |
| Target/Specificity This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MLLT3. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AF9 (MLLT3) Antibody (Center V422) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AF9 (MLLT3) Antibody (Center V422) - Protein Information | |
| Name MLLT3 | |
| Synonyms AF9, YEATS3 | |
| Function Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA | |
| Cellular Location Nucleus (By similarity). | |
AF9 (MLLT3) Antibody (Center V422) - Related products
AP6190a: AF9 (MLLT3) Antibody (C-term K486)
AP6190b: AF9 (MLLT3) Antibody (Center V422)
AP6190c: MLLT3 Antibody (N-term H54)
RI13329: MLLT3 predesign siRNA
BP6190a: MLLT3 Antibody (C-term K486) Blocking Peptide
AF9 (MLLT3) Antibody (Center V422) - Application data
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The anti-MLLT3 Pab (Cat. #AP6190b) is used in Western blot to detect MLLT3 in mouse cerebellum tissue lysate
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MLLT3 Antibody (C-term) (Cat. #AP6190b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MLLT3 antibody detected the MLLT3 protein (arrow).
AF9 (MLLT3) Antibody (Center V422) - Research Areas
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BACKGROUND
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23). The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified; BCR1 is within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8. Several different structural elements have been identified in AF9, including a colocalizing in vivo DNA topo II cleavage site and an in vitro DNase I hypersensitive (DNase 1 HS) site in intron 7 in BCR2. Reversibility experiments demonstrated a religation of the topo II cleavage sites. In addition, 2 scaffold associated regions (SARs) are located centromeric to the topo II and DNase I HS cleavage sites and border breakpoint regions in 2 leukemic cells lines: SAR1 is located in intron 4, whereas SAR2 encompasses parts of exons 5-7. The patient breakpoint regions of AF9 share the same structural elements as the MLL BCR. A DNA breakage and repair model for nonhomologous recombination between MLL and its partner genes, particularly AF9, has been proposed.
REFERENCES
Iida, S., et al., Oncogene 8(11):3085-3092 (1993). Nakamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 90(10):4631-4635 (1993). Strissel, P. L., et al., Hum. Molec. Genet. 9: 1671-1679 (2000).
