- CITATIONS: 3
|Application ||WB, IHC-P, IF, E|
|Calculated MW||54002 Da|
|Antigen Region||391-420 aa|
|Other Names||Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME|
|Target/Specificity||This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Found in alveolar macrophages but not in peripheral blood monocytes|
Provided below are standard protocols that you may find useful for product applications.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.
Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001).
Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).
Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).
Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).
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