|Application ||WB, IHC-P, E|
|Calculated MW||53820 Da|
|Antigen Region||295-324 aa|
|Other Names||Collagenase 3, 3424-, Matrix metalloproteinase-13, MMP-13, MMP13|
|Target/Specificity||This MMP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MMP13.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP13 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.|
|Cellular Location||Secreted, extracellular space, extracellular matrix. Secreted|
|Tissue Location||Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.|
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Provided below are standard protocols that you may find useful for product applications.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Roy-Beaudry, M., et al., Arthritis Rheum. 48(10):2855-2864 (2003).
Liacini, A., et al., Exp. Cell Res. 288(1):208-217 (2003).
Hantke, B., et al., Biol. Chem. 384(8):1247-1251 (2003).
Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003).
Tardif, G., et al., Osteoarthr. Cartil. 11(7):524-537 (2003).
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