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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NOTCH3 Antibody (C-term)   

NOTCH3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NOTCH3 Antibody (C-term) AP6220a
    NOTCH3-Q2306 (Cat. #AP6220a) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).
  • IF - NOTCH3 Antibody (C-term) AP6220a
    Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term)(Cat#AP6220a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • IHC-P - NOTCH3 Antibody (C-term) AP6220a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UM47
Reactivity Mouse
Predicted Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 243631 Da
Antigen Region 2291-2321 aa
Additional Information
Gene ID 4854
Other Names Neurogenic locus notch homolog protein 3, Notch 3, Notch 3 extracellular truncation, Notch 3 intracellular domain, NOTCH3
Target/Specificity This NOTCH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOTCH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Ubiquitously expressed in fetal and adult tissues
Research Areas
Citations ( 0 )


NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).


Suwanwela, N., et al., J Med Assoc Thai 86(2):178-182 (2003). Ahearn, E.P., et al., Am. J. Med. Genet. 114(6):652-658 (2002). Bellavia, D., et al., Proc. Natl. Acad. Sci. U.S.A. 99(6):3788-3793 (2002). Ito, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(3):382-384 (2002). Joutel, A., et al., Lancet 350(9090):1511-1515 (1997).

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$ 295.00
$ 99.00
Cat# AP6220a
(40 western blots)
Availability: In Stock
Bulk Size
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