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Presenilin 1 (PSEN1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Presenilin 1 (PSEN1) Antibody (C-term) AP6231A
    All lanes : Anti-PSEN1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Presenilin 1 (PSEN1) Antibody (C-term) AP6231A
    The Presenilin 1 (PSEN1) Antibody (C-term) (Cat.#AP6231a) is used in Western blot to detect PSEN1 in mouse kidney tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
  • IHC-P - Presenilin 1 (PSEN1) Antibody (C-term) AP6231A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • FC - Presenilin 1 (PSEN1) Antibody (C-term) AP6231A
    Presenilin 1 (PSEN1) Antibody (C-term) (Cat. #AP6231a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - Presenilin 1 (PSEN1) Antibody (C-term) AP6231A
    Confocal immunofluorescent analysis of Presenilin 1 (PSEN1) Antibody (C-term)(Cat#AP6231a) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P49768
Other Accession P97887, P49769, Q8HXW5
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52668 Da
Antigen Region 330-359 aa
Additional Information
Gene ID 5663
Other Names Presenilin-1, PS-1, 3423-, Protein S182, Presenilin-1 NTF subunit, Presenilin-1 CTF subunit, Presenilin-1 CTF12, PS1-CTF12, PSEN1, AD3, PS1, PSNL1
Target/Specificity This Presenilin 1 (PSEN1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the C-terminal region of human Presenilin 1 (PSEN1).
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPresenilin 1 (PSEN1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSEN1
Synonyms AD3, PS1, PSNL1
Function Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:15274632, PubMed:10545183, PubMed:10593990, PubMed:10206644, PubMed:10899933, PubMed:10811883, PubMed:12679784, PubMed:12740439, PubMed:25043039, PubMed:26280335). Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed:15274632, PubMed:25043039, PubMed:26280335). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:9738936, PubMed:10593990, PubMed:10899933, PubMed:10811883). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11953314). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:9738936, PubMed:11953314). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Cytoplasmic granule. Cell membrane Note=Translocates with bound NOTCH1 from the endoplasmic reticulum and/or Golgi to the cell surface (PubMed:10593990). Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane (PubMed:9738936). Also present in azurophil granules of neutrophils (PubMed:11987239). Colocalizes with UBQLN1 in the cell membrane and in cytoplasmic juxtanuclear structures called aggresomes (PubMed:21143716).
Tissue Location Detected in azurophile granules in neutrophils and in platelet cytoplasmic granules (at protein level) (PubMed:11987239). Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes (PubMed:7596406, PubMed:8641442, PubMed:8574969)
Research Areas
Citations ( 0 )

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes.

References

Marambaud, P., et al., Cell 114(5):635-645 (2003). Kim, S.H., et al., J. Biol. Chem. 278(36):33992-34002 (2003). Miklossy, J., et al., Neurobiol. Aging 24(5):655-662 (2003). Cai, D., et al., J. Biol. Chem. 278(5):3446-3454 (2003). Godin, C., et al., Neuroreport 14(12):1613-1616 (2003).

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$ 295.00
$ 99.00
Cat# AP6231A
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(40 western blots)
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