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SIRT3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SIRT3 Antibody (C-term) AP6242a
    Anti-SIRT3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SIRT3 Antibody (C-term) AP6242a
    All lanes : Anti-SIRT3 Antibody (C-term) at 1:2000 dilution Lane 1: human kidney lysate Lane 2: human liver lysate Lane 3: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SIRT3 Antibody (C-term) AP6242a
    Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term)(Cat. #AP6242a). AP6242a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - SIRT3 Antibody (C-term) AP6242a
    SIRT3 Antibody (C-term) (Cat.# AP6242a) western blot analysis in 293 cell lysate (35ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow).
  • WB - SIRT3 Antibody (C-term) AP6242a
    SIRT3 Antibody (C-term) (Cat.# AP6242a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
  • WB - SIRT3 Antibody (C-term) AP6242a
    SIRT3 Antibody (C-term) (Cat.# AP6242a) western blot analysis in KG-1 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
  • WB - SIRT3 Antibody (C-term) AP6242a
    Western blot analysis of SIRT3 antibody (Cat. #AP6242a) in Y79 cell line lysates (35ug/lane). SIRT3 (arrow) was detected using the purified Pab.
  • WB - SIRT3 Antibody (C-term) AP6242a
    Sirt3 Antibody (G265) (Cat. #AP6242a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow).
  • IHC-P - SIRT3 Antibody (C-term) AP6242a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • SPECIFICATION
  • CITATIONS: 10
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NTG7
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43573 Da
Antigen Region 250-279 aa
Additional Information
Gene ID 23410
Other Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIRT3, SIR2L3
Target/Specificity This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT3
Synonyms SIR2L3
Function NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Cellular Location Mitochondrion matrix
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )

Background

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

References

Hirschey, M.D., et al. Nature 464(7285):121-125(2010) Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010) Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)

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$ 295.00
$ 99.00
Cat# AP6242a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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