- CITATIONS: 2
|Application ||WB, IHC-P, E|
|Calculated MW||36615 Da|
|Antigen Region||304-334 aa|
|Other Names||NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3|
|Target/Specificity||This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||M Sirt3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||NAD-dependent protein deacetylase (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:23835326, PubMed:17923681, PubMed:18794531, PubMed:21172655). Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O (PubMed:16790548, PubMed:18794531, PubMed:21172655). Contributes to the regulation of the cellular energy metabolism (PubMed:23835326). Important for regulating tissue-specific ATP levels (PubMed:18794531, PubMed:24252090). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).|
|Cellular Location||Isoform L: Mitochondrion matrix|
|Tissue Location||Strongly expressed in liver and kidney (PubMed:11056054). Expressed in skeletal muscles (at protein level) (PubMed:23283301, PubMed:23835326). Weakly expressed in lung (PubMed:11056054).|
Provided below are standard protocols that you may find useful for product applications.
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Hirschey, M.D., et al. Nature 464(7285):121-125(2010)
Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010)
Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.