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ST5 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ST5 Antibody (C-term) AP6249a
    ST5 Antibody (C-term K1121) (Cat. #AP6249a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).
    detail
  • FC - ST5 Antibody (C-term) AP6249a
    ST5 Antibody (C-term) (Cat. #AP6249a) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P78524
Other Accession Q924W7, NP_005409
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 126485 Da
Antigen Region 1106-1137 aa
Additional Information
Gene ID 6764
Other Names Suppression of tumorigenicity 5 protein, DENN domain-containing protein 2B, HeLa tumor suppression 1, ST5, DENND2B, HTS1
Target/Specificity This ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1106-1137 amino acids from the C-terminal region of human ST5.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsST5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DENND2B (HGNC:11350)
Function [Isoform 1]: May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane (PubMed:29030480).
Cellular Location [Isoform 1]: Cytoplasm, cell cortex. Cell membrane. Recycling endosome. Note=Colocalizes with RAB13 and ITSN1 at cytoplasmic vesicles that are most likely recycling endosomes Colocalizes with the cortical actin cytoskeleton
Tissue Location Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level)
Research Areas
Citations (0)
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Background

ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.

References

Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000).
Hubbs, A.E., et al., Oncogene 18(15):2519-2525 (1999).
Majidi, M., et al., J. Biol. Chem. 273(26):16608-16614 (1998).
Lichy, J.H., et al., Nucleic Acids Res. 24(23):4700-4708 (1996).
Lichy, J.H., et al., Cell Growth Differ. 3(8):541-548 (1992).

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$ 182.50
$ 70.00
Cat# AP6249a
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