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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   CD82 (ST6) Antibody (C-term)   

CD82 (ST6) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CD82 (ST6) Antibody (C-term) AP6250a
    Anti-ST6 Antibody (C253) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CD82 (ST6) Antibody (C-term) AP6250a
    All lanes : Anti-ST6 Antibody (C253) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CD82 (ST6) Antibody (C-term) AP6250a
    Western blot analysis of ST6 (arrow) using ST6 Antibody (C-term) (Cat.#AP6250a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2) (Origene Technologies).
  • IHC-P - CD82 (ST6) Antibody (C-term) AP6250a
    Formalin-fixed and paraffin-embedded human lymph with CD82 (ST6) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CD82 (ST6) Antibody (C-term) AP6250a
    Flow cytometric analysis of HepG2 cells using CD82 (ST6) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27701
Other Accession NP_002222
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29626 Da
Antigen Region 239-267 aa
Additional Information
Gene ID 3732
Other Names CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27, CD82, CD82, KAI1, SAR2, ST6, TSPAN27
Target/Specificity This CD82 (ST6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-267 amino acids from the C-terminal region of human CD82 (ST6).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD82 (ST6) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD82
Synonyms KAI1, SAR2, ST6, TSPAN27
Function Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Lymphoid specific.
Research Areas
Citations (0)

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The ST6 metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients.


Zhang, X.A., et al., J. Biol. Chem. 278(29):27319-27328 (2003).
Zhang, X.A., et al., Cancer Res. 63(10):2665-2674 (2003).
Yang, J., et al., Ai Zheng 22(5):533-536 (2003).
Sauer, G., et al., Oncol. Rep. 10(2):405-410 (2003).
Ito, Y., et al., Pathol Res Pract 199(2):79-83 (2003).

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$ 295.00
$ 99.00
Cat# AP6250a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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