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27Kip1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6269b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
27Kip1 Antibody (C-term) - Product info | |
| Application | WB, IF
|
| Primary Accession | P46527 |
| Other Accession | Q60439 |
| Reactivity | Human |
| Predicted | Hamster |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 22073 Da |
27Kip1 Antibody (C-term) - Additional info | |
| Gene ID 1027 | |
| Other Names CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1 | |
| Target/Specificity This 27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human 27Kip1. | |
| Dilution WB~~1:50~100WB~~1:1000 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions 27Kip1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
27Kip1 Antibody (C-term) - Protein Information | |
| Name CDKN1B | |
| Synonyms KIP1 | |
| Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry | |
| Cellular Location Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) | |
| Tissue Location Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney | |
27Kip1 Antibody (C-term) - Related products
AP10418a: 27Kip1-T157 Antibody
AP13302b: CDKN1B Antibody (C-term)
AP3191a: Phospho-p27Kip1-S10 Antibody
AP3192a: Phospho-p27Kip1-S178 Antibody
AP3193a: Phospho-p27Kip1-T157 Antibody
AP3194a: Phospho-p27Kip1-T187 Antibody
AP3529a: Phospho-p27Kip1-T198 Antibody
AP6269b: 27Kip1 Antibody (C-term)
RI10983: CDKN1B predesign siRNA
DC00893: Human CDKN1B cDNA Clone
LY12364a: CDKN1B Over-expression Lysate
BP10418a: 27Kip1-T157 Antibody Blocking peptide
BP3191a: Phospho-p27Kip1-S10 Antibody Blocking Peptide
BP3192a: Phospho-p27Kip1-S178 Antibody Blocking Peptide
BP3193a: Phospho-p27Kip1-T157 Antibody Blocking Peptide
BP3194a: Phospho-p27Kip1-T187 Antibody Blocking Peptide
BP3529a: Phospho-p27Kip1-T198 Antibody Blocking Peptide
BP6269b: 27Kip1 Antibody (C-term) Blocking Peptide
AT1481a: CDKN1B Antibody (monoclonal) (M01)
AJ1569c: p27/Kip1 Antibody (C-term)
AJ1569d: p27/Kip1 Antibody Phospho (pS10)
27Kip1 Antibody (C-term) - Application data
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Western blot analysis of p27Kip1 Antibody (C-term)(Cat. #6269b) in HepG2 cell line lysates (35ug/lane). 27Kip1(arrow) was detected using the purified Pab (1:60 dilution).
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Confocal immunofluorescent analysis of 27Kip1 Antibody (C-term)(Cat#AP6269b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
27Kip1 Antibody (C-term) - Research Areas
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BACKGROUND
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
REFERENCES
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005). Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005). Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
