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Cdc6 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Cdc6 Antibody (N-term) AP6271a
    Western blot analysis of Cdc6 Antibody (N-term)(Cat.# AP6271a) in Romas cell line lysates (35ug/lane). Cdc6 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99741
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62720 Da
Antigen Region 32-61 aa
Additional Information
Gene ID 990
Other Names Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L
Target/Specificity This Cdc6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human Cdc6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCdc6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC6
Synonyms CDC18L
Function Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Cellular Location Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells
Research Areas
Citations (0)

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Human Cdc6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.


Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004).
Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003).
Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003).
Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002).
Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).

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Cat# AP6271a
(40 western blots)
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