|Application ||WB, E|
|Other Accession||Q8QFR2, Q61666, P79987|
|Predicted||Chicken, Mouse, Xenopus|
|Calculated MW||111835 Da|
|Antigen Region||431-461 aa|
|Other Names||Protein HIRA, TUP1-like enhancer of split protein 1, HIRA, DGCR1, HIR, TUPLE1|
|Target/Specificity||This HIRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431-461 amino acids from the Central region of human HIRA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIRA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DGCR1, HIR, TUPLE1|
|Function||Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.|
|Cellular Location||Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence|
|Tissue Location||Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this protein in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. It plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate protein in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Zhang,R., Mol. Cell. Biol. 27 (6), 2343-2358 (2007)
Tang,Y., Nat. Struct. Mol. Biol. 13 (10), 921-929 (2006)
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