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ELK1 Antibody (S383)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ELK1 Antibody (S383) AP6282A
    Western blot analysis of ELK1 (arrow) using rabbit polyclonal ELK1 Antibody (S383) (RB11172). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELK1 gene (Lane 2) (Origene Technologies).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P19419
Other Accession P41969, A4GTP4
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44888 Da
Antigen Region 361-390 aa
Additional Information
Gene ID 2002
Other Names ETS domain-containing protein Elk-1, ELK1
Target/Specificity This ELK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from human ELK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELK1 Antibody (S383) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELK1
Function Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK- signaling pathway stimulation (By similarity).
Cellular Location Nucleus.
Tissue Location Lung and testis.
Research Areas
Citations (0)
citation

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Background

ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.

References

Salinas, S., et al., J. Cell Biol. 165(6):767-773 (2004).
Yang, S.H., et al., Mol. Cell 12(1):63-74 (2003).
Bebien, M., et al., Oncogene 22(12):1836-1847 (2003).
Sharrocks, A.D., Biochem. Soc. Trans. 30(2):1-9 (2002).
Murai, K., et al., Mol. Cell. Biol. 22(20):7083-7092 (2002).

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$ 182.50
$ 70.00
Cat# AP6282A
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