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Parp12 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Parp12 Antibody (N-term) AP6298a
    Parp12 Antibody (N-term) (Cat.#AP6298a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Parp12 antibody detected the Parp12 protein (arrow).
  • IHC-P - Parp12 Antibody (N-term) AP6298a
    Parp12 Antibody (N-term) (Cat. #AP6298a)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parp12 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - Parp12 Antibody (N-term) AP6298a
    Parp12 Antibody (N-term) (Cat. #AP6298a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BZ20
Other Accession NP_766481
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79917 Da
Antigen Region 312-344 aa
Additional Information
Gene ID 243771
Other Names Poly [ADP-ribose] polymerase 12, PARP-12, ADP-ribosyltransferase diphtheria toxin-like 12, ARTD12, Zinc finger CCCH domain-containing protein 1, Parp12, Zc3hdc1
Target/Specificity This Parp12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-344 amino acids from the N-terminal region of human Parp12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsParp12 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Parp12
Synonyms Zc3hdc1
Cellular Location Nucleus.
Citations (0)

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Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.


Bailey,P.J., Exp. Cell Res. 312 (16), 3108-3119 (2006)
Katoh,M., Int. J. Oncol. 23 (2), 541-547 (2003)

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Cat# AP6298a
(40 western blots)
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