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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   APH1 Antibody (N-term)   

APH1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - APH1 Antibody (N-term) AP6302A
    Western blot analysis of anti-APH1 Antibody (N-term) (Cat. #AP6302a) in A2058 cell line lysates (35ug/lane). APH1 (arrow) was detected using the purified Pab.
  • IHC-P - APH1 Antibody (N-term) AP6302A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term) (Cat.#AP6302a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96BI3
Other Accession Q8BVF7
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28996 Da
Antigen Region 77-109 aa
Additional Information
Gene ID 51107
Other Names Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, APH1A, PSF
Target/Specificity This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human APH1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APH1A
Synonyms PSF
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi
Tissue Location Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Research Areas
Citations (0)

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Background

APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

References

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).
Kimberly, W.T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6382-6387 (2003).
Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).

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Cat# AP6302A
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(40 western blots)
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