|Application ||WB, IHC-P, E|
|Calculated MW||28996 Da|
|Antigen Region||77-109 aa|
|Other Names||Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, APH1A, PSF|
|Target/Specificity||This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human APH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi- pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi|
|Tissue Location||Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).
Kimberly, W.T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(11):6382-6387 (2003).
Edbauer, D., et al., Nat. Cell Biol. 5(5):486-488 (2003).
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