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ABAD Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ABAD Antibody (C-term) AP6307b
    Western blot analysis of anti-ABAD Pab (Cat. #AP6307b) in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab.
    detail
  • WB - ABAD Antibody (C-term) AP6307b
    ABAD Antibody (C214) (Cat. #AP6307b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ABAD antibody detected the ABAD protein (arrow).
    detail
  • IHC-P - ABAD Antibody (C-term) AP6307b
    Immunohistochemistry on Formalin-fixed and paraffin-embedded human prostate tissue. (Top)Coloration with the Anti-ABAD antibody at a 1:20 concentration. (Bottom)Antibody adsorbed to the antigen (peptide), negative control.Disappearance of cytoplasmic staining indicating the antibody is specific. Data and protocol courtesy of Marie-Hélène Levesque, Centre de Recherche du CHUL, Canada.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q6IBS9
Other Accession O70351, O08756, Q99714, O02691
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 199-235 aa
Additional Information
Other Names hydroxyacyl-Coenzyme A dehydrogenase, type II isoform 1; 3-hydroxyacyl-CoA dehydrogenase type II; Type II HADH; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; Endoplasmic reticulum-associated amyloid beta-peptide binding protein; Short-chain type dehydrogenase/reductase XH98G2 ; ERAB, HSD17B10, SCHAD; HADH2 protein
Target/Specificity This ABAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-235 amino acids from the C-terminal region of human ABAD.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABAD Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)
citation

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Background

Amyloid b-peptide-binding alcohol dehydrogenase (ABAD) is a member of the family of short chain dehydrogenase/reductases; unique among this family, it binds amyloid b-peptide and exhibits enzymatic activity toward a wide variety of substrates including linear alcohols. In an amyloid beta-abundant environment, ABAD appears to trigger cell stress induced by the amyloid peptide.

References

FASEB J. 19 (6), 597-598 (2005) J. Mol. Biol. 342 (3), 943-952 (2004) Science 304 (5669), 448-452 (2004) FEBS Lett. 451 (3), 238-242 (1999) J. Biol. Chem. 274 (21), 15014-15019 (1999)

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$ 365.00
$ 140.00
Cat# AP6307b
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