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CLC4 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | P51793 |
| Other Accession | P51794, Q61418 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84917 Da |
| Antigen Region | 663-689 aa |
| Gene ID | 1183 |
|---|---|
| Other Names | H(+)/Cl(-) exchange transporter 4, Chloride channel protein 4, ClC-4, Chloride transporter ClC-4, CLCN4 |
| Target/Specificity | This CLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-689 amino acids from the C-terminal region of human CLC4. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P-Leica~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLC4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLCN4 |
|---|---|
| Function | Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:18063579, PubMed:28972156, PubMed:23647072, PubMed:27550844, PubMed:25644381). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874). |
| Cellular Location | Early endosome membrane {ECO:0000250|UniProtKB:P51794}; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Note=Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3 |
| Tissue Location | Abundant in skeletal muscle and also detectable in brain and heart |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders.
References
Wang, T., et al., Gastroenterology 126(4):1157-1166 (2004).
Lamb, F.S., et al., J. Mol. Cell. Cardiol. 31(3):657-666 (1999).
Dinulos, M.B., et al., Genomics 35(1):244-247 (1996).
Schnur, R.E., et al., Hum. Genet. 95(5):594-595 (1995).
van Slegtenhorst, M.A., et al., Hum. Mol. Genet. 3(4):547-552 (1994).
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