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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   NURR1 (NR4A2) Antibody (N-term)   

NURR1 (NR4A2) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NURR1 (NR4A2) Antibody (N-term) AP6412a
    Western blot analysis of anti-NURR1(NR4A2) Pab (Cat. #AP6412a) in mouse brain tissue lysate. NURR1(NR4A2) (arrow) was detected using the purified Pab.
  • IHC-P - NURR1 (NR4A2) Antibody (N-term) AP6412a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with NURR1 (NR4A2) antibody (N-term)(Cat#AP6412a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - NURR1 (NR4A2) Antibody (N-term) AP6412a
    Confocal immunofluorescent analysis of NURR1 (NR4A2) Antibody (N-term) (Cat#AP6412a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - NURR1 (NR4A2) Antibody (N-term) AP6412a
    NR4A2 Antibody (N-term) (Cat. #AP6412a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR4A2 antibody detected the NR4A2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P43354
Other Accession Q07917, Q06219, Q08E53
Reactivity Human, Mouse
Predicted Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 66591 Da
Antigen Region 13-42 aa
Additional Information
Gene ID 4929
Other Names Nuclear receptor subfamily 4 group A member 2, Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor, NR4A2, NOT, NURR1, TINUR
Target/Specificity This NURR1 (NR4A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human NURR1 (NR4A2).
Dilution WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNURR1 (NR4A2) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR4A2
Synonyms NOT, NURR1, TINUR
Function Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity).
Cellular Location Cytoplasm. Nucleus. Note=Mostly nuclear; oxidative stress promotes cytoplasmic localization
Tissue Location Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue
Research Areas
Citations ( 0 )

Background

Parkinson's disease (PD) is a multifactorial disease that appears to arise from the effects of both genetic and environmental influences. The known genetic factors include multiple genes that have been identified in related parkinsonian syndromes, as well as alpha-synuclein. Genes associated with either PD or Parkinson-related disorders include parkin, DJ-1, ubiquitin C-terminal hydrolase isozyme L1 (UCH-L1), nuclear receptor-related factor 1 (NURR1), and alpha-synuclein. Nurr1 is a transcription factor that is expressed in the embryonic ventral midbrain and is critical for the development of dopamine (DA) neurons. It belongs to the conserved family of nuclear receptors but lacks an identified ligand and is therefore referred to as an orphan receptor. RXR ligands can promote the survival of DA neurons via a process that depends on Nurr1-RXR heterodimers. In developing DA cells, Nurr1 is required for the expression of several genes important for DA synthesis and function. Nurr1 is also important for the maintenance of adult DA neurons.

References

Perlmann T, et al. Cell Tissue Res. 318(1):45-52 (2004) Hsu HC,et al. Curr Drug Targets Inflamm Allergy. 3(4):413-23 (2004) Wallen-Mackenzie A, et al. Genes Dev. 17(24):3036-47 (2003) Ichinose,H., et al. Gene 230 (2), 233-239 (1999) Okabe,T., et al. J. Immunol. 154 (8), 3871-3879 (1995) Mages,H.W., et al. Mol. Endocrinol. 8 (11), 1583-1591 (1994)

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$ 295.00
$ 99.00
Cat# AP6412a
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(40 western blots)
Availability: In Stock
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