|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||157263 Da|
|Antigen Region||1361-1391 aa|
|Other Names||Copper-transporting ATPase 2, Copper pump 2, Wilson disease-associated protein, WND/140 kDa, ATP7B, PWD, WC1, WND|
|Target/Specificity||This ATP7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1361-1391 amino acids from the C-terminal region of human ATP7B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP7B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||PWD, WC1, WND|
|Function||Copper ion transmembrane transporter involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Multi- pass membrane protein. Late endosome Note=Predominantly found in the trans-Golgi network (TGN) Localized in the trans-Golgi network under low copper conditions, redistributes to cytoplasmic vesicles when cells are exposed to elevated copper levels, and then recycles back to the trans-Golgi network when copper is removed (PubMed:10942420). Not redistributed to the plasma membrane in response to elevated copper levels. Isoform 2: Cytoplasm|
|Tissue Location||Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver The cleaved form WND/140 kDa is found in liver cell lines and other tissues|
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Provided below are standard protocols that you may find useful for product applications.
ATP7B is a member of the P-type cation transport ATPase family and a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile.
Martinez-Balibrea,E., Int. J. Cancer 124 (12), 2905-2910 (2009)
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