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FLI1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - FLI1 Antibody (Center) AP6514c
    Confocal immunofluorescent analysis of FLI1 Antibody (Center)(Cat#AP6514c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
    detail
  • WB - FLI1 Antibody (Center) AP6514c
    Western blot analysis of FLI1 antibody (Center) (Cat.# AP6514c) in mouse lung tissue lysates(35ug/lane). FLI1 (arrow) was detected using the purified Pab.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q01543
Other Accession P41157, P26323, Q29RS8, O70132, Q8QZW2, Q99581, A3FEM2, P29774, P81270, P11308, Q90837
Reactivity Human, Mouse
Predicted Chicken, Drosophila, Zebrafish, Rat, Bovine, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50982 Da
Antigen Region 299-328 aa
Additional Information
Gene ID 2313
Other Names Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Target/Specificity This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-328 amino acids from the Central region of human FLI1.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLI1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FLI1
Function Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'- C[CA]GGAAGT-3'.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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Background

FLI1 is a sequence-specific transcriptional activator. It recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

References

Embree,L.J., Cancer Res. 69 (10), 4363-4371 (2009)
Torlakovic,E.E., Histol. Histopathol. 23 (11), 1309-1314 (2008)
Cironi,L., PLoS ONE 3 (7), E2634 (2008)

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$ 365.00
$ 140.00
Cat# AP6514c
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