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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GCG Antibody (N-term)   

GCG Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GCG Antibody (N-term) AP6515A
    Western blot analysis of GCG antibody (N-term) (Cat.# AP6515a) in mouse bladder tissue lysates (35ug/lane). GCG (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GCG Antibody (N-term) AP6515A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GCG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - GCG Antibody (N-term) AP6515A
    Flow cytometric analysis of HepG2 cells using GCG Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession P01275
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20909 Da
Antigen Region 25-54 aa
Additional Information
Gene ID 2641
Other Names Glucagon, Glicentin, Glicentin-related polypeptide, GRPP, Oxyntomodulin, OXM, OXY, Glucagon, Glucagon-like peptide 1, GLP-1, Incretin hormone, Glucagon-like peptide 1(7-37), GLP-1(7-37), Glucagon-like peptide 1(7-36), GLP-1(7-36), Glucagon-like peptide 2, GLP-2, GCG
Target/Specificity This GCG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human GCG.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCG (HGNC:4191)
Function [Glucagon]: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
Cellular Location Secreted.
Tissue Location [Glucagon]: Secreted in the A cells of the islets of Langerhans. [Glucagon-like peptide 2]: Secreted from enteroendocrine cells throughout the gastrointestinal tract. Also secreted in selected neurons in the brain [Oxyntomodulin]: Secreted from enteroendocrine cells throughout the gastrointestinal tract
Research Areas
Citations (0)
citation

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Background

GCG is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.

References

Jacobo,S.M., J. Pharmacol. Exp. Ther. 330 (1), 283-293 (2009)
Root-Bernstein,R.J. Mol. Recognit. 22 (3), 177-187 (2009)

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$ 365.00
$ 140.00
Cat# AP6515A
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