|Application ||WB, IHC-P, E|
|Calculated MW||76690 Da|
|Antigen Region||462-488 aa|
|Other Names||NADPH--cytochrome P450 reductase, CPR, P450R, POR, CYPOR|
|Target/Specificity||This P450R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 462-488 amino acids from the Central region of human P450R.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||P450R Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.|
|Cellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Anchored to the ER membrane by its N- terminal hydrophobic region|
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Provided below are standard protocols that you may find useful for product applications.
P450R is an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in its gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Fukami,M., J. Clin. Endocrinol. Metab. 94 (5), 1723-1731 (2009)
Mast,N., Arch. Biochem. Biophys. 483 (1), 81-89 (2009)
Brenner,S., FEBS J. 275 (18), 4540-4557 (2008)
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