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ARPC1A Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # Size Availability Price  
AP6519b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP6519b-ev20 Ev20 size 100 ul In Stock $ 95.00 Add to cart
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ARPC1A Antibody (C-term) - Product info

ApplicationWB, IHC, IF, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionQ92747
Other AccessionQ99PD4, Q9R0Q6, Q1JP79
ReactivityHuman
PredictedBovine, Mouse, Rat
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW41569 Da

ARPC1A Antibody (C-term) - Additional info

Gene ID 10552
Other Names
ARPC1A; SOP2L; Actin-related protein 2/3 complex subunit 1A; SOP2-like protein
Target/Specificity
This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293~323 amino acids from the C-terminal region of human ARPC1A.
Dilution
WB~~1:100~500
IHC~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ARPC1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

ARPC1A Antibody (C-term) - Protein Information

Name ARPC1A
Synonyms SOP2L
Function
Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks
Cellular Location
Cytoplasm, cytoskeleton (Probable).

ARPC1A Antibody (C-term) - Related products

AP6519b: ARPC1A Antibody (C-term)

AP6519c: ARPC1A Antibody (Center)

RI10411: ARPC1A predesign siRNA

DC07286: Human ARPC1A cDNA Clone

LY11814a: ARPC1A Over-expression Lysate

BP6519b: ARPC1A Antibody (C-term) Blocking Peptide

BP6519c: ARPC1A Antibody (Center) Blocking Peptide

ARPC1A Antibody (C-term) - Application data

  • Western blot analysis of ARPC1A Antibody (C-term) (Cat. #AP6519b) in Hela cell line lysates (35ug/lane). ARPC1A (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

  • Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ARPC1A Antibody (C-term) - Research Areas

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BACKGROUND

ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

REFERENCES

Laurila,E., Genes Chromosomes Cancer 48 (4), 330-339 (2009) Machesky,L.M., Biochem. J. 328 (PT 1), 105-112 (1997)