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ARPC1A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ARPC1A Antibody (C-term) AP6519B
    Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
    detail
  • WB - ARPC1A Antibody (C-term) AP6519B
    Anti-ARPC1A Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ARPC1A Antibody (C-term) AP6519B
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ARPC1A Antibody (C-term) AP6519B
    Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IF, IHC-P, WB, E
Primary Accession Q92747
Other Accession Q99PD4, Q9R0Q6, Q1JP79, Q8AVT9, A0A1L8EXB5
Reactivity Human
Predicted Xenopus, Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41569 Da
Antigen Region 286-315 aa
Additional Information
Gene ID 10552
Other Names Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
Target/Specificity This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARPC1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARPC1A
Synonyms SOP2L
Function Probably functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Cellular Location Cytoplasm, cytoskeleton. Nucleus {ECO:0000250|UniProtKB:Q8AVT9}
Research Areas
Citations (0)
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Background

ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.

References

Laurila,E., Genes Chromosomes Cancer 48 (4), 330-339 (2009)
Machesky,L.M., Biochem. J. 328 (PT 1), 105-112 (1997)

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$ 365.00
$ 140.00
Cat# AP6519B
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