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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   CLNS1A Antibody (C-term)   

CLNS1A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CLNS1A Antibody (C-term) AP6520b
    Western blot analysis of CLNS1A antibody (C-term) (Cat.# AP6520b) in HL60 cell line lysates (35ug/lane). CLNS1A (arrow) was detected using the purified Pab.
    detail
  • WB - CLNS1A Antibody (C-term) AP6520b
    All lanes : Anti-CLNS1A Antibody (C-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CLNS1A Antibody (C-term) AP6520b
    Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CLNS1A Antibody (C-term) AP6520b
    Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term)(bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession P54105
Other Accession Q28678
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26215 Da
Antigen Region 189-216 aa
Additional Information
Gene ID 1207
Other Names Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, I(Cln), Chloride ion current inducer protein, ClCI, Reticulocyte pICln, CLNS1A, CLCI, ICLN
Target/Specificity This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the C-terminal region of human CLNS1A.
Dilution WB~~1:8000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLNS1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLNS1A
Synonyms CLCI, ICLN
Function Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins (PubMed:21081503, PubMed:18984161, PubMed:10330151, PubMed:11713266). Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre- mRNAs (PubMed:18984161, PubMed:10330151). Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:10330151). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:10330151, PubMed:18984161). Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (PubMed:10330151, PubMed:18984161).
Cellular Location Cytoplasm, cytosol. Nucleus. Cytoplasm, cytoskeleton. Note=A small fraction is also associated with the cytoskeleton (PubMed:18984161)
Research Areas
Citations (0)
citation

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Background

CLNS1A is a protein that functions in multiple regulatory pathways. The protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator.

References

Yeung,C.H., Biol. Reprod. 73 (5), 1057-1063 (2005)

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$ 365.00
$ 140.00
Cat# AP6520b
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