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ACTG1 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6532c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
ACTG1 Antibody (Center) - Product info | |
| Application | WB, FC, IF
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| Primary Accession | P63261 |
| Other Accession | P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709, P48975; |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Drosophila, C.Elegans, Hamster, Mouse, Pig, Monkey, Rat, Rabbit, Xenopus, Yeast, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 41793 Da |
ACTG1 Antibody (Center) - Additional info | |
| Gene ID 71 | |
| Other Names ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin; Actin, cytoplasmic 2, N-terminally processed | |
| Target/Specificity This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~224 amino acids from the Center region of human ACTG1. | |
| Dilution WB~~1:50~100WB~~1:1000 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACTG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACTG1 Antibody (Center) - Protein Information | |
| Name ACTG1 | |
| Synonyms ACTB, ACTG | |
| Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells | |
| Cellular Location Cytoplasm, cytoskeleton. | |
ACTG1 Antibody (Center) - Related products
AP6532a: ACTG1 Antibody (N-term)
AP6532c: ACTG1 Antibody (Center)
RI10088: ACTG1 predesign siRNA
DC00125: Human ACTG1 cDNA Clone
LY10837a: ACTG1 Over-expression Lysate
ACTG1 Antibody (Center) - Application data
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Western blot analysis of ACTG1 Antibody (Center) (Cat.# AP6532c) in K562, HepG2 cell line lysates (35ug/lane). ACTG1 (arrow) was detected using the purified Pab.
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Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody (Center) (Cat. #AP6532c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.
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Confocal immunofluorescent analysis of ACTG1 Antibody (Center)(Cat#AP6532c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ACTG1 Antibody (Center) - Research Areas
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BACKGROUND
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.
REFERENCES
de Heer,A.M., Ann. Otol. Rhinol. Laryngol. 118 (5), 382-390 (2009) Mouilleron,S., EMBO J. 27 (23), 3198-3208 (2008) Liu,P., J Genet Genomics 35 (9), 553-558 (2008)
