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CRAT Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P43155 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 70858 Da |
| Antigen Region | 66-96 aa |
| Gene ID | 1384 |
|---|---|
| Other Names | Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CAT, CrAT, CRAT, CAT1 |
| Target/Specificity | This CRAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human CRAT. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CRAT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CRAT (HGNC:2342) |
|---|---|
| Synonyms | CAT1 |
| Function | Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta- oxidation (PubMed:15099582, PubMed:29395073). Responsible for the synthesis of short- and branched-chain acylcarnitines (PubMed:23485643). Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates (PubMed:23485643). Trans-2- enoyl-CoAs and 2-methylacyl-CoAs are poor substrates (PubMed:23485643). |
| Cellular Location | Endoplasmic reticulum. Peroxisome. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side [Isoform 2]: Peroxisome |
| Tissue Location | Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
CRAT is carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments.
References
Govindasamy,L., J. Struct. Biol. 146 (3), 416-424 (2004)
Jogl,G., Cell 112 (1), 113-122 (2003)
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