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Calponin-3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Calponin-3 Antibody (N-term) AP6581A
    Western blot analysis of Calponin-3 Antibody (N-term) (Cat. #AP6581a) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - Calponin-3 Antibody (N-term) AP6581A
    Calponin-3 Antibody (N-term) (Cat. #AP6581a) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - Calponin-3 Antibody (N-term) AP6581A
    Calponin-3 Antibody (N-term) (Cat. #AP6581a) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q15417
Other Accession Q32L92
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 36414 Da
Antigen Region 7-34 aa
Additional Information
Gene ID 1266
Other Names Calponin-3, Calponin, acidic isoform, CNN3
Target/Specificity This Calponin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human Calponin-3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalponin-3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNN3
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue Location Expressed in both non-smooth muscle tissues as well as smooth muscle tissues
Research Areas
Citations (0)
citation

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Background

Calponin-3 is a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. This protein is associated with the cytoskeleton but is not involved in contraction.

References

Vinatzer,U., Clin. Cancer Res. 14 (20), 6426-6431 (2008)
Haag,J., Exp. Cell Res. 313 (16), 3386-3394 (2007)

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$ 365.00
$ 140.00
Cat# AP6581A
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