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COL6A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - COL6A1 Antibody (C-term) AP6587B
    Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody (C-term)(Cat. #AP6587b). AP6587b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - COL6A1 Antibody (C-term) AP6587B
    Western blot analysis of COL6A1 antibody (C-term) (Cat. #AP6587b) in Y79 cell line lysates (35ug/lane). COL6A1 (arrow) was detected using the purified Pab.
  • IHC-P - COL6A1 Antibody (C-term) AP6587B
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with COL6A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - COL6A1 Antibody (C-term) AP6587B
    Confocal immunofluorescent analysis of COL6A1 Antibody (C-term)(Cat#AP6587b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - COL6A1 Antibody (C-term) AP6587B
    Flow cytometric analysis of 293 cells using COL6A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12109
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 108529 Da
Antigen Region 831-858 aa
Additional Information
Gene ID 1291
Other Names Collagen alpha-1(VI) chain, COL6A1
Target/Specificity This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-858 amino acids from the C-terminal region of human COL6A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL6A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL6A1
Function Collagen VI acts as a cell-binding protein.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)

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The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein COL6A1 is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.


Martoni,E., Hum. Mutat. 30 (5), E662-E672 (2009)
Kim,T.H., J. Rheumatol. 35 (9), 1849-1852 (2008)
Fujita,A., Genet. Mol. Res. 7 (2), 371-378 (2008)

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Cat# AP6587B
(40 western blots)
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