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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   EEF1A1 Antibody (C-term)   

EEF1A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EEF1A1 Antibody (C-term) AP6592b
    Western blot analysis of EEF1A1 Antibody (C-term) (Cat. #AP6592b) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.
  • FC - EEF1A1 Antibody (C-term) AP6592b
    Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - EEF1A1 Antibody (C-term) AP6592b
    Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term)(Cat#AP6592b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • IHC-P - EEF1A1 Antibody (C-term) AP6592b
    EEF1A1 Antibody (C-term) (AP6592b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68104
Other Accession Q92005, Q90835, Q5VTE0, P62632, Q71V39, P62631, Q05639, Q32PH8, P62630, P68105, P10126, P62629, P68103, P13549, A2Q0Z0
Reactivity Human
Predicted Xenopus, Bovine, Hamster, Mouse, Rabbit, Rat, Chicken, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50141 Da
Antigen Region 361-389 aa
Additional Information
Gene ID 1915
Other Names Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7
Target/Specificity This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1A1
Synonyms EEF1A, EF1A, LENG7
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens
Tissue Location Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
Research Areas
Citations (0)

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EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.


Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)

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$ 295.00
$ 99.00
Cat# AP6592b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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