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WNT10B Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WNT10B Antibody (Center) AP6595b
    All lanes : Anti-WNT10B Antibody (Center) at 1:2000 dilution Lane 1: Rat skeletal muscle whole lysate Lane 2: Human skeletal muscle whole lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - WNT10B Antibody (Center) AP6595b
    WNT10B Antibody (Center) (Cat. #AP6595b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - WNT10B Antibody (Center) AP6595b
    Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O00744
Other Accession P48614
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43000 Da
Antigen Region 193-222 aa
Additional Information
Gene ID 7480
Other Names Protein Wnt-10b, Protein Wnt-12, WNT10B, WNT12
Target/Specificity This WNT10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human WNT10B.
Dilution WB~~1:2000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT10B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT10B
Synonyms WNT12
Function Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted
Tissue Location Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain
Research Areas
Citations (0)
citation

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Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.

References

Zmuda,J.M., J. Bone Miner. Res. 24 (3), 437-447 (2009)
Pederson,L., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20764-20769 (2008)

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$ 365.00
$ 140.00
Cat# AP6595b
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