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ARF3 Antibody (Center D93)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P61204 |
| Other Accession | P61206, P61205, Q5E9I6, P84082, Q8BSL7, P84081, P51643, P84079, P84078, Q4R5P2, P84077, P84080 |
| Reactivity | Human |
| Predicted | Bovine, Monkey, Mouse, Rat, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20601 Da |
| Antigen Region | 78-106 aa |
| Gene ID | 377 |
|---|---|
| Other Names | ADP-ribosylation factor 3, ARF3 |
| Target/Specificity | This ARF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-106 amino acids from the Central region of human ARF3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARF3 Antibody (Center D93) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARF3 |
|---|---|
| Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. |
| Cellular Location | Golgi apparatus. Cytoplasm, perinuclear region |
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Provided below are standard protocols that you may find useful for product applications.
Background
ADP-ribosylation factor 3 (ARF3) is a member of the human ARF family. These small guanine nucleotide-binding proteins stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.
References
Li,F., FEBS Lett. 524 (1-3), 49-53 (2002)
Lee,F.J., J. Biol. Chem. 267 (34), 24441-24445 (1992)
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