RNGTT Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | O60942 |
Other Accession | O55236 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 68557 Da |
Antigen Region | 489-516 aa |
Gene ID | 8732 |
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Other Names | mRNA-capping enzyme, HCAP1, HCE, Polynucleotide 5'-triphosphatase, mRNA 5'-triphosphatase, TPase, mRNA guanylyltransferase, GTP--RNA guanylyltransferase, GTase, RNGTT, CAP1A |
Target/Specificity | This RNGTT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-516 amino acids from the C-terminal region of human RNGTT. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNGTT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNGTT |
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Synonyms | CAP1A |
Function | Bifunctional mRNA-capping enzyme exhibiting RNA 5'- triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate. |
Cellular Location | Nucleus. |
Tissue Location | Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. The protein catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
References
Zhou,M., Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671 (2003)
Chiu,Y.L., J. Biol. Chem. 276 (16), 12959-12966 (2001)
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