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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GLO1 Antibody (N-term)   

GLO1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GLO1 Antibody (N-term) AP6649a
    All lanes : Anti-GLO1 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GLO1 Antibody (N-term) AP6649a
    Western blot analysis of GLO1 antibody (N-term) (Cat. #AP6649a) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.
  • IHC-P - GLO1 Antibody (N-term) AP6649a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - GLO1 Antibody (N-term) AP6649a
    Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - GLO1 Antibody (N-term) AP6649a
    Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) (Cat. #AP6649a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.
  • IF - GLO1 Antibody (N-term) AP6649a
    Confocal immunofluorescent analysis of GLO1 Antibody (N-term)(Cat#AP6649a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q04760
Other Accession Q4R5F2
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20778 Da
Antigen Region 23-51 aa
Additional Information
Gene ID 2739
Other Names Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, GLO1
Target/Specificity This GLO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human GLO1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLO1
Function Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Required for normal osteoclastogenesis.
Research Areas
Citations (0)

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The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.


Germanova,A., Cancer Invest. 27 (6), 655-660 (2009)
Engelen,L., J. Hypertens. 27 (7), 1399-1403 (2009)

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$ 295.00
$ 99.00
Cat# AP6649a
(40 western blots)
Availability: In Stock
Bulk Size
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