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HPX Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HPX Antibody (Center) AP6653c
    All lanes : Anti-HPX Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Human plasma lysate Lane 3: Mouse plasma lysate Lane 4: Rat plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HPX Antibody (Center) AP6653c
    All lanes : Anti-HPX Antibody (Center) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - HPX Antibody (Center) AP6653c
    Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP6653c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - HPX Antibody (Center) AP6653c
    Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP6653c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - HPX Antibody (Center) AP6653c
    HPX Antibody (Center) (Cat. #AP6653c) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, FC, E
Primary Accession P02790
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51676 Da
Antigen Region 200-227 aa
Additional Information
Gene ID 3263
Other Names Hemopexin, Beta-1B-glycoprotein, HPX
Target/Specificity This HPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the Central region of human HPX.
Dilution WB~~1:2000
IHC-P-Leica~~1:500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHPX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HPX
Function Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Cellular Location Secreted.
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
Citations (0)
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Background

HPX is a plasma glycoprotein that binds heme with high affinity. The protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.

References

Ascenzi,P., IUBMB Life 59 (11), 700-708 (2007)

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$ 365.00
$ 140.00
Cat# AP6653c
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