NKRF Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | O15226 |
Other Accession | Q8BY02 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 77673 Da |
Antigen Region | 205-233 aa |
Gene ID | 55922 |
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Other Names | NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4, Transcription factor NRF, NKRF, ITBA4, NRF |
Target/Specificity | This NKRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-233 amino acids from the Central region of human NKRF. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NKRF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NKRF {ECO:0000303|PubMed:32179686, ECO:0000312|HGNC:HGNC:19374} |
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Function | Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Also involved in the regulation of IL-8 transcription (PubMed:12381793). May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553). |
Cellular Location | Nucleus, nucleolus. |
Tissue Location | Widely and constitutively expressed (PubMed:10562553). Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney (PubMed:10562553) |
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Provided below are standard protocols that you may find useful for product applications.
Background
NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.
References
Ho,S.C., J. Immunol. 183 (1), 411-420 (2009)
Glanz,A., Immunol. Cell Biol. 86 (7), 622-630 (2008)
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