|Application ||WB, IHC-P, E|
|Calculated MW||77673 Da|
|Antigen Region||205-233 aa|
|Other Names||NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4, Transcription factor NRF, NKRF, ITBA4, NRF|
|Target/Specificity||This NKRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-233 amino acids from the Central region of human NKRF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NKRF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.|
|Cellular Location||Nucleus, nucleolus|
|Tissue Location||Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney|
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Provided below are standard protocols that you may find useful for product applications.
NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.
Ho,S.C., J. Immunol. 183 (1), 411-420 (2009)
Glanz,A., Immunol. Cell Biol. 86 (7), 622-630 (2008)
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