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NUP155 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NUP155 Antibody (N-term) AP6661a
    Western blot analysis of NUP155 antibody (N-term) (Cat. #AP6661a) in HL60 cell line lysates (35ug/lane). NUP155 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - NUP155 Antibody (N-term) AP6661a
    (LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with NUP155 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using NUP155 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O75694
Other Accession P37199, Q99P88
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 155199 Da
Antigen Region 9-38 aa
Additional Information
Gene ID 9631
Other Names Nuclear pore complex protein Nup155, 155 kDa nucleoporin, Nucleoporin Nup155, NUP155, KIAA0791
Target/Specificity This NUP155 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human NUP155.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUP155 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUP155
Synonyms KIAA0791
Function Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
Cellular Location Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P37199}. Nucleus membrane {ECO:0000250|UniProtKB:P37199}; Peripheral membrane protein {ECO:0000250|UniProtKB:P37199}; Cytoplasmic side {ECO:0000250|UniProtKB:P37199}. Nucleus membrane {ECO:0000250|UniProtKB:P37199}; Peripheral membrane protein {ECO:0000250|UniProtKB:P37199}; Nucleoplasmic side {ECO:0000250|UniProtKB:P37199}. Note=In mitosis, assumes a diffuse cytoplasmic distribution probably as a monomer, before reversing back into a punctate nuclear surface localization at the end of mitosis {ECO:0000250|UniProtKB:P37199}
Tissue Location Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)
citation

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Background

Nucleoporins are the main components of the nuclear pore complex (NPC) of eukaryotic cells. They are involved in the bidirectional trafficking of molecules, especially mRNAs and proteins, between the nucleus and the cytoplasm. The protein does not contain the typical FG repeat sequences found in most vertebrate nucleoporins.

References

Zhang,X., Cell 135 (6), 1017-1027 (2008)
Rayala,H.J., Mol. Cell Proteomics 3 (2), 145-155 (2004)

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$ 182.50
$ 70.00
Cat# AP6661a
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