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SCFD1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SCFD1 Antibody (C-term) AP6672b
    Western blot analysis of SCFD1 antibody (C-term) (Cat. #AP6672b) in MCF-7 cell line lysates (35ug/lane). SCFD1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - SCFD1 Antibody (C-term) AP6672b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with SCFD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - SCFD1 Antibody (C-term) AP6672b
    Flow cytometric analysis of MDA-MB468 cells using SCFD1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q8WVM8
Other Accession Q62991, Q8BRF7
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 72380 Da
Antigen Region 543-572 aa
Additional Information
Gene ID 23256
Other Names Sec1 family domain-containing protein 1, SLY1 homolog, Sly1p, Syntaxin-binding protein 1-like 2, SCFD1, C14orf163, KIAA0917, STXBP1L2
Target/Specificity This SCFD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human SCFD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCFD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCFD1
Synonyms C14orf163, KIAA0917, STXBP1L2
Function Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein Golgi apparatus, Golgi stack membrane; Peripheral membrane protein
Research Areas
Citations (0)
citation

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Background

SCFD1 is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

References

Matsuo,N., J. Biol. Chem. 272 (26), 16438-16444 (1997)

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$ 182.50
$ 70.00
Cat# AP6672b
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