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Sestrin-2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Sestrin-2 Antibody (Center) AP6674c
    Western blot analysis of Sestrin-2 Antibody (Center) (Cat. #AP6674c) in 293 cell line lysates (35ug/lane). Sestrin-2 (arrow) was detected using the purified Pab. .
  • FC - Sestrin-2 Antibody (Center) AP6674c
    Sestrin-2 Antibody (Center) (Cat. #AP6674c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P58004
Other Accession P58043
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54494 Da
Antigen Region 283-311 aa
Additional Information
Gene ID 83667
Other Names Sestrin-2, Hi95, SESN2, SEST2
Target/Specificity This Sestrin-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the Central region of human Sestrin-2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSestrin-2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SESN2 (HGNC:20746)
Function Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612, PubMed:26449471, PubMed:26612684, PubMed:26586190). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed:26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (PubMed:19113821).
Cellular Location Cytoplasm.
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

SESN2 is a member of the sestrin family of PA26-related proteins. The protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.

References

Budanov,A.V., Science 304 (5670), 596-600 (2004)
Peeters,H., Hum. Genet. 112 (5-6), 573-580 (2003)

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Cat# AP6674c
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(40 western blots)
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